Figure 3

Comparison of recombinant strains containing 1, 2, 4, and 8 gene copies of prml. A: After 96 hours incubation at 30°C, mα-2pRML-X33 displayed higher enzyme activity (1200 U/mL), specific activity (1684 U/mg), and protein concentration (0.770 mg/mL) in the fermentation supernatant. B: rml transcription level in the 1-, 2-, 4, and 8-copy strains. At both 48 and 96 hours, transcription level was highest in the 4-copy strain and lower in the 8-copy strain.