Detection of esterification reaction products by GC and TLC. A: GC detection. The substrate (microalgae oil) more than 90% conversion to biodiesel (FAME) after esterification reaction for 24 hours at 30°C. There was no significant difference between reactions catalyzed by a single lipase (Lipase GH2) vs. a combination of two lipases (Lipase GH2 and MDL). B: TLC detection. Lane: 1-monooleate (MAG). Lane 2: dioleoylglycerol (1,3- and 1,2-diacylglycerol). Lane 3: oleic acid (FFA). Lane 4: triacylglycerol (TAG). Lanes 5–6: single-lipase (Lipase GH2) treatment at 0 h and 24 h. Lanes 7–8: two-lipase (Lipase GH2 and MDL) treatment at 0 and 24 hours.