Table 2 Plasmids and strains used in this study
| Â | Description | Source |
|---|---|---|
Plasmids | ||
pPICZα A | Secretion expression vector with α-factor from S. cerevisiae | Donated by Dr Yijun Huang |
pPICMα A | pPICZα A with α-factor optimized codons | Donated by Dr Jia Ban |
Strains | ||
X-33 | Host strain (WT Mut+) | Donated by Dr Yijun Huang |
zα-1mRML-X33 | One copy mRML of the lipase without propeptide expressed in X-33 using pPICZα A | This study |
zα-1pRML-X33 | One copy pPML of the lipase with propeptide expressed in X-33 using pPICZα A | This study |
mα-1pRML-X33 | One copy pRML expressed in X-33 using pPICMα A | This study |
mα-2pRML-X33 | Two copies pRML expressed in X-33 using pPICMα A | This study |
mα-4pRML-X33 | Four copies pRML expressed in X-33 using pPICMα A | This study |
mα-8pRML-X33 | Eight copies pRML expressed in X-33 using pPICMα A | This study |