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Table 2 Plasmids and strains used in this study

From: Improved production of a recombinant Rhizomucor miehei lipase expressed in Pichia pastoris and its application for conversion of microalgae oil to biodiesel

  Description Source
Plasmids
pPICZα A Secretion expression vector with α-factor from S. cerevisiae Donated by Dr Yijun Huang
pPICMα A pPICZα A with α-factor optimized codons Donated by Dr Jia Ban
Strains
X-33 Host strain (WT Mut+) Donated by Dr Yijun Huang
zα-1mRML-X33 One copy mRML of the lipase without propeptide expressed in X-33 using pPICZα A This study
zα-1pRML-X33 One copy pPML of the lipase with propeptide expressed in X-33 using pPICZα A This study
mα-1pRML-X33 One copy pRML expressed in X-33 using pPICMα A This study
mα-2pRML-X33 Two copies pRML expressed in X-33 using pPICMα A This study
mα-4pRML-X33 Four copies pRML expressed in X-33 using pPICMα A This study
mα-8pRML-X33 Eight copies pRML expressed in X-33 using pPICMα A This study