Table 1 Kinetic constants for oxidation of five substrates (at fixed pH for each substrate) by C. subvermispora MnP6 and its S168W and S168W-environment variants compared with P. chrysosporium LiP-H8 and P. ostreatus VP1
From: Engineering a fungal peroxidase that degrades lignin at very acidic pH
MnP6 | S168W | S168W-environment | LiP-H8 | VP1 | ||
|---|---|---|---|---|---|---|
Mn2+ | K m (μM) | 8.7 ± 1.6 | 8.5 ± 1.2 | 11.0 ± 1.8 | - | 98.0 ± 5.6 |
kcat (s-1) | 83.0 ± 5.0 | 60.5 ± 5.0 | 66.9 ± 6.0 | 0 | 185.0 ± 2.6 | |
kcat/K m (s-1.mM-1) | 9540 ± 650 | 7110 ± 650 | 6060 ± 650 | 0 | 1900 ± 90 | |
VA | K m (μM) | 0 | 2740 ± 560 | 21600 ± 200 | 190 ± 17 | 5500 ± 46 |
kcat (s-1) | 0 | 0.54 ± 0.04 | 1.69 ± 0.04 | 17.5 ± 0.5 | 12.7 ± 0.5 | |
kcat/K m (s-1. mM-1) | - | 0.197 ± 0 | 0.078 ± 0 | 92.0 ± 6.0 | 2.3 ± 0.2 | |
RB5 | K m (μM) | 0 | 12.6 ± 3 | 5.4 ± 3 | 0 | 5.4 ± 0.2 |
kcat (s-1) | 0 | 7.8 ± 1.1 | 7.0 ± 1.1 | 0 | 12.9 ± 0.3 | |
kcat/K m (s-1. mM-1) | - | 619 ± 27 | 1310 ± 100 | - | 2380 ± 50 | |
ABTS | K m (μM) | 0 | 60.2 ± 13.4 | 0 | 23.0 ± 1.6 | 4.0 ± 0.4 |
kcat (s-1) | 0 | 1.4 ± 0.1 | 0 | 13.0 ± 0.2 | 14.4 ± 0.4 | |
kcat/K m (s-1. mM-1) | - | 23 ± 3 | - | 563 ± 36 | 3600 ± 20 | |
DMP | K m (μM) | 0 | 0 | 0 | 5.8 ± 0.5 | 54 ± 4 |
kcat (s-1) | 0 | 0 | 0 | 10.0 ± 0.1 | 6.6 ± 0.1 | |
kcat/K m (s-1. mM-1) | - | - | - | 1720 ± 153 | 122 ± 7 |