Table 2 Kinetic constants for VA and RB5 oxidation by the S164W variant of C. subvermispora MnP6 at acidic pH values
From: Engineering a fungal peroxidase that degrades lignin at very acidic pH
pH 1.6 | pH 2.0 | pH 3.0 | ||
|---|---|---|---|---|
VA | K m (μM) | 650 ± 97 | 913 ± 52 | 2740 ± 560 |
kcat (s-1) | 1.35 ± 0.06 | 1.08 ± 0.01 | 0.54 ± 0.04 | |
kcat/K m (s-1. mM-1) | 2.08 ± 0.24 | 1.08 ± 0.06 | 0.197 ± 0 | |
pH 2.5 | pH 3.5 | |||
RB5 | K m (μM) | 1.4 ± 0.2 | 12.6 ± 3 | |
kcat (s-1) | 12.9 ± 0.7 | 7.8 ± 1.1 | ||
kcat/K m (s-1. mM-1) | 9050 ± 930 | 619 ± 27 | ||