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Table 2 Kinetic constants for VA and RB5 oxidation by the S164W variant of C. subvermispora MnP6 at acidic pH values

From: Engineering a fungal peroxidase that degrades lignin at very acidic pH

   pH 1.6 pH 2.0 pH 3.0
VA K m (μM) 650 ± 97 913 ± 52 2740 ± 560
kcat (s-1) 1.35 ± 0.06 1.08 ± 0.01 0.54 ± 0.04
kcat/K m (s-1. mM-1) 2.08 ± 0.24 1.08 ± 0.06 0.197 ± 0
   pH 2.5 pH 3.5
RB5 K m (μM) 1.4 ± 0.2 12.6 ± 3
kcat (s-1) 12.9 ± 0.7 7.8 ± 1.1
kcat/K m (s-1. mM-1) 9050 ± 930 619 ± 27
  1. Means and 95% confidence limits from triplicate measurements of substrate oxidation at 25°C in 100 mM BR buffer of different pH values.