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Table 3 Kinetic constants for degradation of a lignin model dimer by the S168W variant of C. subvermispora MnP6 (pH 1.6) and P. ostreatus VP1 (pH 3.0) (S168W oxidation at pH 3.0 is also shown)

From: Engineering a fungal peroxidase that degrades lignin at very acidic pH

   S168W S168W VP1
pH 1.6 pH 3.0 pH 3.0
Lignin dimer K m (μM) 1.1 ± 0.2 4.9 ± 1.3 1.7 ± 0.1
  kcat (s-1) 0.47 ± 0.0 0.36 ± 0.1 0.49 ± 0.0
kcat/K m (s-1.mM-1) 0.42 ± 0.0 0.07 ± 0.0 0.28 ± 0.0
  1. Means and 95% confidence limits from triplicate measurements of 4-O-methyl-syringylglycerol-β-guaiacyl ether degradation at 25°C in 100 mM BR buffer of pH 1.6 (only S168W) and pH 3.0.