Table 3 Kinetic constants for degradation of a lignin model dimer by the S168W variant of C. subvermispora MnP6 (pH 1.6) and P. ostreatus VP1 (pH 3.0) (S168W oxidation at pH 3.0 is also shown)
From: Engineering a fungal peroxidase that degrades lignin at very acidic pH
S168W | S168W | VP1 | ||
|---|---|---|---|---|
pH 1.6 | pH 3.0 | pH 3.0 | ||
Lignin dimer | K m (μM) | 1.1 ± 0.2 | 4.9 ± 1.3 | 1.7 ± 0.1 |
kcat (s-1) | 0.47 ± 0.0 | 0.36 ± 0.1 | 0.49 ± 0.0 | |
kcat/K m (s-1.mM-1) | 0.42 ± 0.0 | 0.07 ± 0.0 | 0.28 ± 0.0 |