Figure 1

Expression and zymogram of MpXyn10A in A. nidulans. (A) SDS-PAGE analysis of the culture filtrate recovered from flask cultures; 1-4) Four different transformants of A. nidulans carrying the MpXyn10A ORF after 5% maltose induction for 48 h at 37°C, under static conditions. L) ladder; A773 + pEXPYR) A. nidulans A773 transformed with empty pEXPYR after 5% maltose induction for 48 h at 37°C, under static conditions. (B) SDS-PAGE of purified MpXyn10A. (C ) Zymogram of the crude extract of A. nidulans expressing MpXyn10A (Xyn10A) and of A. nidulans transformed with empty pEXPYR (A773 + pEXPYR) after 5% maltose induction for 48 h at 37°C. See Methods section for further details.