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Figure 2 | Biotechnology for Biofuels

Figure 2

From: A novel thermostable xylanase GH10 from Malbranchea pulchella expressed in Aspergillus nidulans with potential applications in biotechnology

Figure 2

Effect of temperature and pH and the thermal stability of MpXyn10A. (A) Effect of temperature on xylanase activity. The activity was measured on incubating xylan birchwood (substrate) in 50 mM sodium acetate buffer, pH 5.0, at the tested temperatures for 10 min. (B) Effect of pH on xylanase activity (from 3.5 to 8.0). The activity was measured upon incubating xylan birchwood (substrate) in 100 mM sodium citrate/phosphate buffer at the different pH values tested at 80°C for 10 min. (C) Xylanase activity thermostability. Black triangles 80°C; black circles 70°C; black diamonds 65°C. The activity was measured upon incubating xylan birchwood (substrate) in 50 mM sodium acetate buffer, pH 5.5, at 80°C for 10 min. In all experiments the detection of reducing sugars released was conducted as previously described by Miller [33].

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