The effect of glycosylation on MpXyn10A and structural analyses. (A) Analyses of MpXyn10A glycosylation by SDS-PAGE. L - ladder; 1 - MpXyn10A treated with endoglycosidase H (Endo H) and purified; 2 - untreated MpXyn10A. (B) Differential scanning fluorimetry of glycosylated (solid squares) and deglycosylated (open circles) MpXyn10A. The melting temperature was estimated at 80.34°C for the glycosylated enzyme, with R2 = 0.9956, and 80.13°C for the deglycosylated enzyme, with R2 = 0.9975. (C) Three-dimensional structure of MpXyn10A generated by molecular modeling. Black arrows indicate the predicted glycosylation sites. (D) Far-UV circular dichroism spectrum of MpXyn10A. See Methods section for further experimental details.