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Table 2 Xylanase activities of MpXyn10A and wild-type Malbranchea pulchella against birchwood and oat spelt xylan as substrates

From: A novel thermostable xylanase GH10 from Malbranchea pulchella expressed in Aspergillus nidulans with potential applications in biotechnology

  MpXyn10A Malbranchea pulchella
Xylanase activity Birchwood Oat spelt Birchwood Oat spelt
U/mL 5.867 ± 0.102 6.175 ± 0.301 2.985 ± 0.051 3.946 ± 0.030
U/μg of protein 838.3 ± 7.1 882.1 ± 10.1 99.5 ± 3.2 131.5 ± 1.4
  1. This experiment was conducted by incubating the MpXyn10A with 1% xylan in 50 mM sodium acetate buffer, pH 5.5, at 80°C for 10 min. The amount of reducing sugars released was quantified by the Miller method [33].