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Table 2 Xylanase activities of MpXyn10A and wild-type Malbranchea pulchella against birchwood and oat spelt xylan as substrates

From: A novel thermostable xylanase GH10 from Malbranchea pulchella expressed in Aspergillus nidulans with potential applications in biotechnology

 

MpXyn10A

Malbranchea pulchella

Xylanase activity

Birchwood

Oat spelt

Birchwood

Oat spelt

U/mL

5.867 ± 0.102

6.175 ± 0.301

2.985 ± 0.051

3.946 ± 0.030

U/μg of protein

838.3 ± 7.1

882.1 ± 10.1

99.5 ± 3.2

131.5 ± 1.4

  1. This experiment was conducted by incubating the MpXyn10A with 1% xylan in 50 mM sodium acetate buffer, pH 5.5, at 80°C for 10 min. The amount of reducing sugars released was quantified by the Miller method [33].
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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