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Table 3 Test of the CBM1 functionality

From: A novel thermostable xylanase GH10 from Malbranchea pulchella expressed in Aspergillus nidulans with potential applications in biotechnology

  Supernatant Bound to the polysaccharide
  Glycosylated Deglycosylated Glycosylated Deglycosylated
  Avicel Oat spelt Avicel Oat spelt Avicel Oat spelt Avicel Oat spelt
Relative activity (%) 21.5 ± 2.3 88.9 ± 3.9 27.2 ± 2.1 85.2 ± 4.2 78.5 ± 3.3 11.1 ± 2.8 72.8 ± 5.1 14.8 ± 3.1
  1. Glycosylated or deglycosylated MpXyn10A was incubated with Avicel or oat spelt xylan for 30 min at 4°C under agitation in 50 mM sodium acetate buffer, pH 5.5. The supernatant was separated from the polysaccharide, and the xylanolytic activity of each fraction was measured by incubation with 0.5% birchwood xylan at 70°C for 10 min. The amount of reducing sugars released was measured by the Miller method [33].