Figure 1

Growth, microscopy and flow cytometry. (A) Growth curves of S. cerevisiae (square), C. curvatus (triangle), R. toruloides (circle) in rich malt extract, soytone (MS) medium (30 g/l malt extract, 3 g/l soytone). (B) S. cerevisiae C. curvatus and R. toruloides cells were stained with Nile red (9-diethylamino-5H-benzo[α]phenoxazine-5-one) after 72 h (T72) of growth and observed under a microscope. (C) Flow cytometric analysis of S. cerevisiae, C. curvatus and R. toruloides after 0 (T0) and 72 h after inoculum in rich medium. Cells were washed twice and stained for 5 minutes with Nile red before flow cytometric analysis. X mean, values representing the medium fluorescence of S. cerevisiae C. curvatus, and R. toruloides. OD, optical density.