Figure 4

Cellulase and xylanase production by T. reesei QM9414 recombinant strains overexpressing the candidate regulatory genes. The volumetric enzyme production (blue bars) and production per biomass dry weight (red bars) are shown as the fold change of the maximum amount of activity produced in the cultures of the recombinant strains as compared to the maximum activity produced in the cultures of the parental strain. The values are means of three biological replicates. Error bars show the standard error of the mean. Panels A and B show the total xylanase activity against birch glucuronoxylan substrate and cellulase activity against 4-methylumbelliferyl-β-D-lactoside substrate, respectively. Panels C and D show the specific enzymatic activity produced by cellobiohydrolase 1 and endoglucanase 1. Detailed time course data on enzyme production in the cultures is shown in the Additional file 2. CBHI, cellobiohydrolase 1; EGI, endoglucanase 1; MUL, 4-methylumbelliferyl-β-D-lactoside.