Figure 7

Quantitative PCR analysis of cellulase and hemicellulase gene expression of strains overexpressing the constructs pMH15, pMH20 and pMH25. Panels A, B and C show the expression levels of the analyzed genes for strains overexpressing the constructs pMH15, pMH20 and pMH25, respectively. Expression levels are normalized against the signal of sar1 and are shown as a fold change as compared to the normalized expression level in the parental strain. RNA extracted after 3 days (blue bars) and 5 days (red bars) of cultivation was used as a template. The values are means of three biological replicates. Error bars show the standard error of the mean.