Quantitative PCR analysis of cellulase and hemicellulase gene expression by the strains overexpressing gene 77513 and by the strain with gene 77513 deleted. pMH15 is harboring the expression cassette as a double-copy and pMH15(S) as a single-copy. Panels A, B, C, D, E and F show the expression levels of cbh1, cbh2, egl1, bgl1, bxl1 and axe1 genes, respectively. Expression levels are shown as normalized against the signal of sar1. RNA extracted after 3 days (blue bars) and 5 days (red bars) of cultivation was used as a template. The values are means of three biological replicates. Error bars show the standard error of the mean.