Figure 9From: Screening of candidate regulators for cellulase and hemicellulase production in Trichoderma reesei and identification of a factor essential for cellulase productionQuantitative PCR analysis of cellulase and hemicellulase gene expression by the strains overexpressing gene 77513 and by the strain with gene 77513 deleted. pMH15 is harboring the expression cassette as a double-copy and pMH15(S) as a single-copy. Panels A, B, C, D, E and F show the expression levels of cbh1, cbh2, egl1, bgl1, bxl1 and axe1 genes, respectively. Expression levels are shown as normalized against the signal of sar1. RNA extracted after 3 days (blue bars) and 5 days (red bars) of cultivation was used as a template. The values are means of three biological replicates. Error bars show the standard error of the mean.Back to article page