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Table 1 Predicted cellulase enzymes identified in the switchgrass-adapted metagenome
| Gene ID | IMG gene ID | GH family | Predicted function | Max identity (%) | Genbank accession number | Top Blast-hit organism | Metagenome bin* |
|---|---|---|---|---|---|---|---|
| J01 | 2061974227 | 3 | β-glucosidase | 42 | ZP_06970881.1 | Ktedonobacter racemifer DSM 44963 | Paenibacillus |
| J02 | 2061976655 | 3 | β-glucosidase | 97 | YP_003321925.1 | Thermobaculum terrenum | Thermobaculum |
| J03 | 2061976732 | 3 | β-glucosidase | 96 | YP_003322827.1 | Thermobaculum terrenum | Thermobaculum |
| J04 | 2061977694 | 1 | β-glucosidase | 62 | ZP_10205923.1 | Rhodanobacter thiooxydans LCS2 | Gemmatimonadetes |
| J05 | 2061979262 | 3 | β-glucosidase | 44 | YP_002760449.1 | Gemmatimonas aurantiaca T-27 | Gemmatimonadetes |
| J06 | 2061979786 | 1 | β-glucosidase | 61 | ZP_08918778.1 | Thermobacillus composti KWC4 | Paenibacillus |
| J07 | 2061980390 | 1 | β-glucosidase | 66 | NP_242789.1 | Bacillus halodurans C-125 | Not binned |
| J08 | 2062002762 | 1 | β-glucosidase | 99 | YP_003323667.1 | Thermobaculum terrenum ATCC BAA-798 | Not binned |
| J09 | 2062002762 | 1 | β-glucosidase | 98 | YP_003323667.1 | Thermobaculum terrenum ATCC BAA-798 | Not binned |
| J10 | 2062002993 | 3 | β-glucosidase | 77 | ZP_09004353.1 | Paenibacillus lactis 154 | Not binned |
| J11 | 2062005533 | 3 | β-glucosidase | 42 | ZP_06970881.1 | Ktedonobacter racemifer DSM 44963 | Not binned |
| J12 | 2062006736 | 3 | β-glucosidase | 94 | YP_003291338.1 | Rhodothermus marinus DSM 4252 | Rhodothermus1 |
| J13 | 2062007625 | 1 | β-glucosidase | 93 | YP_003318753.1 | Sphaerobacter thermophilus DSM 20745 | Sphaerobacter |
| J14 | 2062008681 | 3 | β-glucosidase | 97 | YP_003324065.1 | Thermobaculum terrenum ATCC BAA-798 | Sphaerobacter |
| J15 | 2062012385 | 3 | β-glucosidase | 75 | YP_823953.1 | Candidatus Solibacter usitatus Ellin6076 | Not binned |
| J16 | 2062018481 | 3 | β-glucosidase | 100 | YP_004824792.1 | Rhodothermus marinus SG0.5JP17-172 | Rhodothermus1 |
| J17 | 2062019328 | 3 | β-glucosidase | 71 | ZP_08918857.1 | Thermobacillus composti KWC4 | Paenibacillus |
| J18 | 2062019735 | 1 | β-glucosidase | 99 | AAN05441.1 | Thermus sp. IB-21 | Thermus |
| J19 | 2062026722 | 1 | β-glucosidase | 72 | YP_002522957.1 | Thermomicrobium roseum DSM 5159 | Thermomicrobium |
| J21 | 2061975668 | 9 | Endoglucanase | 54 | YP_002759529.1 | Gemmatimonas aurantiaca T-27 | Gemmatimonadetes |
| J22 | 2061976479 | 8 | Endoglucanase | 72 | BAF49077.1 | Paenibacillus sp. W-61 | Paenibacillus |
| J23 | 2061977143 | 5 | Endoglucanase | 32 | ZP_09216417.1 | Gordonia amarae NBRC 15530 | Sphaerobacter2 |
| J24 | 2061979932 | 9 | Endoglucanase | 54 | ACJ68032.1 | Paenibacillus provencensis | Paenibacillus |
| J25 | 2061986269 | 12 | Endoglucanase | 98 | YP_004824941.1 | Rhodothermus marinus SG0.5JP17-172 | Rhodothermus2 |
| J26 | 2061990001 | 12 | Endoglucanase | 100 | YP_004824941.1 | Rhodothermus marinus SG0.5JP17-172 | Not binned |
| J27 | 2061990054 | 5 | Endoglucanase | 35 | ZP_09309733.1 | Rhodococcus pyridinivorans AK37 | Sphaerobacter2 |
| J28 | 2061994288 | 5 | Endoglucanase | 98 | YP_003323917.1 | Thermobaculum terrenum ATCC BAA-798 | Sphaerobacter |
| J29 | 2062006179 | 5 | Endoglucanase | 52 | BAJ22272.1 | Paenibacillus sp. KSM-N546 | Paenibacillus |
| J30 | 2062016312 | 9 | Endoglucanase | 54 | ZP_08919343.1 | Thermobacillus composti KWC4 | Not binned |
| J31 | 2062017860 | 5 | Endoglucanase | 57 | ZP_08873206.1 | Verminephrobacter aporrectodeae | Not binned |
| J32 | 2062025020 | 5 | Endoglucanase | 96 | YP_003320228.1 | Sphaerobacter thermophilus DSM 20745 | Not binned |
| J33 | 2062027867 | 8 | Endoglucanase | 72 | ZP_04851456.1 | Paenibacillus sp. oral taxon 786 str. D14 | Not binned |
| J34 | 2062029826 | 6 | Endoglucanase | 37 | ZP_06416445.1 | Frankia sp. EUN1f | Thermobaculum |
| J35 | 2062032441 | 5 | Endoglucanase | 35 | ZP_08873206.1 | Verminephrobacter aporrectodeae | Not Binned |
| J36 | 2062035244 | 5 | Endoglucanase | 100 | YP_004823815.1 | Rhodothermus marinus SG0.5JP17-172 | Rhodothermus1 |
| J38 | 2062019306 | 3 | Cellobiohydrolase | 57 | ZP_08918880.1 | Thermobacillus composti KWC4 | Paenibacillus |
| J39 | 2062019306 | 3 | Cellobiohydrolase | 76 | ZP_08918880.1 | Thermobacillus composti KWC4 | Paenibacillus |
