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Table 3 Activity profile of the active glycoside hydrolase enzymes

From: Discovery and characterization of ionic liquid-tolerant thermophilic cellulases from a switchgrass-adapted microbial community

Gene ID

01

02

03

05

06

07

08

09

11

14

15

16

17

18

19

24

25

26

29

30

36

T opt (°C)

45

90

75

70

65

70

90

90

60

70

70

80

60

95

80

55

95

95

65

50

95

Temp (°C) ≥80% activity

45–50

80–90

75

60–75

55–70

65–70

80–90

80–90

55–60

60–75

65–70

75–80

55–60

85–95

70–85

50–60

80–95

85–95

55–70

45–55

75–95

Temp (°C) ≥50% activity

45–55

70–90

70–80

45–80

45–75

60–75

65–90

65–90

45–65

50–75

60–70

70–80

45–60

70–95

60–85

45–65

60–95

75–95

50–70

45–55

55–95

pH opt

6

7

5.5

7

6

6

5

5

6

6

6.5

5

6

6

5.5

6.5

7.5

7.5

7.5

6

6

pH ≥80% activity

6–6.5

5.5–8

4.5–6

6–7.5

5–6.5

6–7.5

4–8

4–8

5–6.5

5.5–7.5

6–7

5–5.5

5 –7

4.5–7

4.5–7.5

5.5–7.5

4–8

5.5–8

5.5–8

5–7

6–7.5

pH ≥50% activity

5–7

4.5–8

4.5–7

5.5–8

4.5–7.5

5.5–8

4–8

4–8

4.5–7

5–8

6–7.5

5–6.5

5–7.5

4–8

4.5–8

5–8

4–8

4–8

5–8

4.5–8

5.5–8

IL% (v/v) ≥100% activity

0

15

40

35

5

10

0

0

10

5

0

35

0

5

10

10

40

30

5

25

35

IL% (v/v) ≥80% activity

0

25

40

40

5

15

5

5

15

10

0

40

0

15

20

10

40

35

10

30

35

IL% (v/v) ≥50% activity

15

35

40

40

5

15

20

20

20

20

0

40

5

30

30

15

40

35

15

35

35

Max activity in IL*

0.68 (5)

1.1 (5)

1.2 (40)

2.1 (15)

1.3 (5)

1.2 (5)

0.87 (5)

0.89 (5)

1.5 (5)

1.2 (5)

0.45 (5)

5 (10)

0.5 (5)

1.2 (5)

1.1 (5)

2.1 (5)

1.9 (15)

2.5 (15)

1.4 (5)

2.5 (15)

2 (25)

  1. Enzyme activity was profiled at temperatures between 45 and 95°C, pH between 4 and 8, and IL concentrations between 0 and 40% (v/v) of [C2mim][OAc]. The temperature and pH that elicited the highest activity is indicated in row Topt and pHopt, respectively. Temperature and pH ranges that permitted greater than 80% and 50% activity are indicated below the optimum value. IL-tolerance is indicated as the maximum concentration of [C2mim][OAc] that permits at least 80% and 50% enzyme activity (i.e. a value of 15 in the 80% row would indicate that 15% (v/v) of [C2mim][OAc] is the maximum concentration of [C2mim][OAc] that can be used to retain at least 80% enzyme activity). Most enzymes showed a steady decline in activity with increasing IL concentrations. *Max activity in IL is reported as the highest fold change of activity in the presence of IL compared to water and the () indicates the IL concentration (v/v) in which that highest activity as achieved. Values less than 1 indicate the enzyme is less active in IL than in water while values greater than 1 indicate the enzyme has increased activity in the presence of IL.