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Table 1 Bacterial strains and plasmids used in this study

From: Deletion of meso-2,3-butanediol dehydrogenase gene bud C for enhanced D-2,3-butanediol production in Bacillus licheniformis

Strains and plasmids Characteristicsa Source or reference
E. coli strains   
DH5α F Φ80d/lac ZΔM15, Δ(lacZYA-argF) U169, recA 1, endA 1, hsdR 17 (rK, mK+), phoA, supE 44, λ, thi-1, gyrA 96, relA 1 Laboratory stock
B. licheniformis strains   
WX-02 CCTCC M208065, wild type Laboratory stock [14]
WX-02 Δbud C bud C knock-out mutant of WX-02 This study
WX-02 Δbud C/pHYbud C plasmid-based bud C complementation strain of WX-02 Δbud C by introduction of pHYbud C, Tcr This study
Plasmids   
T2(2)-ori E. coli-B. licheniformis shuttle vector, oripUC/orits, temperature-sensitive, Kanr Laboratory stock
T2Δbud C T2(2)-ori derivative containing homologous arms for bud C knock-out This study
pHY300PLK E. coli-B. licheniformis shuttle vector, Apr(E. coli), Tcr(E. coli and B. licheniformis) TaKaRa
pHYbud C pHY300PLK derivative containing bud C, P43 promoter and TamyL (amyL terminator), Apr(E. coli), Tcr(E. coli and B. licheniformis) This study
  1. aTcr, tetracycline resistance; Apr, ampicillin resistance; orits, temperature-sensitive Bacillus origin of replication; Kanr, Kanamycin resistance; CCTCC, China Center for Type Culture Collection.