Skip to main content

Table 2 Primers used in this study

From: Deletion of meso-2,3-butanediol dehydrogenase gene bud C for enhanced D-2,3-butanediol production in Bacillus licheniformis

Primer name

aSequence 5′ → 3′

ΔbudC-A-F

CGCGGATCC AAAGCGCATGTTTTAAAAC

ΔbudC-A-R

CCGCCCTCCATATAGAATATAATTTTAAAAATAAACATCTTCTTTCTATAAGTAA

ΔbudC-B-F

ACCAATTACTTATAGAAAGAAGATGTTTATTTTTAAAATTATATTCTATATGGAG

ΔbudC-B-R

GCTCTAGA CCTCGCACTAGTGTATTTTGAAAC

ΔbudC-F

CGAACTCCATGAACTGACAGTC

ΔbudC-R

TTGCTATTTCCTGTTATGACC

P43-budC-TamyL-1

CGCGGATCC TGTCGACGTGCATGCAGG

P43-budC-TamyL-2

CAATTTTTCCAGATACTTTACTCATGTGTACATTCCTCTCTTACCTATA

P43-budC-TamyL-3

TATAGGTAAGAGAGGAATGTACACATGAGTAAAGTATCTGGAAAAATTG

P43-budC-TamyL-4

CGTCCTCTCTGCTCTTCTATCTTTTAATTAAATACCATTCCGCCATC

P43-budC-TamyL-5

GATGGCGGAATGGTATTTAATTAAAAGATAGAAGAGCAGAGAGGACG

P43-budC-TamyL-6

CCGGAATTC GATCACCCGCGATACCGTC

ΔbudC A signal crossover-F

CTTCACATGGACGATCCTAAT

ΔbudC A single crossover-R

TGTTCCTCCGTAAACCGCTAAG

ΔbudC B single crossover-F

CAACCACCCCTATTGAAAGCAT

ΔbudC B single crossover-R

GATACCTGTCCGCCTTTCTCC

  1. aRestriction sites highlighted in bold. Italics stands for the overlap region for splicing by overlapping extension PCR (SOE-PCR).