Figure 4

SDS-PAGE and starch-containing PAGE analyses of FSA. Lane M, marker; lane 1, culture supernatant of the induced transformant harboring FSA; lane 2, eluted protein after the first HisTrap affinity column; lane 3, eluted protein after the second HisTrap affinity column; lane 4, eluted protein after Superdex 200 column; and lane 5, FSA in starch-containing PAGE. FSA, Flavobacteriaceae Sinomicrobium α-amylase.