Skip to main content
Figure 7 | Biotechnology for Biofuels

Figure 7

From: Ligninolytic peroxidase genes in the oyster mushroom genome: heterologous expression, molecular structure, catalytic and stability properties, and lignin-degrading ability

Figure 7

Phylogram of 237 sequences of basidiomycete PODs including the nine sequences from P. ostreatus genome (underlined). POD sequences from 21 genomes (and GenBank) were analyzed. Four main clusters were identified corresponding to LiP (A), MnP-short/VP clusters 1 and 2 (B, C), and MnP-long (D), together with the most ancestral group of GPs and unclustered sequences. Those clusters/subclusters where Pleurotus (PLEOS, P. ostreatus; PLEER, P. eryngii; PLEPU, P. pulmonarius; and PLESA, P. sapidus) sequences are not included were collapsed, with indication of the number and type of sequences included. Two LiP-type enzymes from G. subvermispora representing LiP/VP transition stages [47] are indicated, as well as a Cerrena unicolor MnP (AFK91532) related to P. ostreatus short MnPs, and the unique Trametes cervina LiP (BAD52441). MnP-atypical corresponds to a MnP type with only two acidic residues at the oxidation site [16]. Protein model numbers are provided for the nine P. ostreatus genome sequences, and GenBank references for other six POD sequences. See Ruiz-Dueñas and Martínez [19] for references of other basidiomycete PODs. GP, generic peroxidase; LiP, lignin peroxidase; MnP, manganese peroxidase; POD, class II peroxidase from the superfamily of non-animal (plant-fungal-prokaryotic) peroxidases; VP, versatile peroxidase.

Back to article page