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Figure 2 | Biotechnology for Biofuels

Figure 2

From: Identification and characterization of a galacturonic acid transporter from Neurospora crassa and its application for Saccharomyces cerevisiae fermentation processes

Figure 2

The NCU00988 deletion strain Δ gat-1 is unable to take up D-galacturonic acid (D-GalA). (A) Carbon source-specific growth phenotype. Conidia of N. crassa wild-type (WT) and the ∆gat-1 deletion strain were inoculated into 3-mL cultures containing various 2% carbon sources and incubated at 25°C, 250 rpm in the light. At the indicated times (1.5 to 4.0 days) the mycelia were harvested and the dry weight determined. Bars represent standard deviations (n = 5). The ∆gat-1 strain displayed a specific growth reduction on pectic substrates (polygalacturonic acid (PGA) and pectin from citrus peel). The extent of the biomass reduction is indicated (in % of WT). (B, C) Monosaccharide transport assays. Sucrose pre-grown N. crassa WT and ∆gat-1 mycelia were transferred for 4 h to 0.5% pectin to induce the pectinolytic response and subsequently to the reaction solution containing 90 μM each of the indicated monosaccharides and Vogel’s salts (at pH 5.8). The cultures were incubated in the reaction solution for 30 minutes at 25°C, 250 rpm in the light. Aliquots of the supernatant were taken at regular intervals and the remaining sugar concentrations analyzed by HPAEC-PAD. Bars represent standard deviations (n = 3). ∆gat-1 was found to be unable to transport D-GalA as well as D-glucuronic acid (D-GlcA). Suc, sucrose.

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