Figure 1

Growth and ethanol production of an ethanologenic Synechocystis 6803 strain (Pr) compared to the empty vector control strain (Co) over a period of 18 days in two independent cultivation experiments, A and B, and in three biological replicates each. (A) Growth curves of triplicate cultures during cultivation A. At time points 4 d, 7 d, 11 d and 18 d, samples were taken for RNA preparation and transcriptome analysis. (B) Accumulation of ethanol in the producer strain (Pr) during cultivation A in three biological replicates. (C) Growth curves of triplicate cultures during cultivation B. At time points 14 d and 18 d, samples were taken for northern and immunoblot analysis. (D) Accumulation of ethanol in the Pr during cultivation B in three biological replicates. Growth during cultivation A: Co, 1.08 ± 0.017 optical density (OD) units d^-1; Pr, 0.65 ± 0.021 OD units d^-1. Growth during cultivation B: Co, 1.06 ± 0.010 OD units d^-1; Pr, 0.66 ± 0.085 OD units d^-1. Production during cultivation A: Pr, 0.0338 ± 0.002% ethanol (EtOH) d^-1. Production during cultivation B: Pr, 0.0303 ± 0.002% EtOH d^-1.