Figure 4

Detail of gene expression analysis (microarrays) in Synechocystis 6803 producer and isogenic control strains (wild type) 4 days (time point t1), 7 days (t2), 11 days (t3) and 18 days (t4) after start of the experiment. (A) The genome section containing the phycocyanin operon cpcBAC2C1D is shown. The location of annotated genes is indicated by the blue boxes. The numbers of RNAseq reads from previous transcriptome analyses under standard conditions [11] are plotted for comparison (dark grey, primary reads; light grey, secondary reads). The normalized log₂ expression values obtained by microarray analyses (normalized expression of biological duplicates A1 and A2 in two technical duplicates each) are plotted for each probe as bars in blue (producer A1 and A2, with increasing colour intensity from t_o to t₄) or green (control incubation, with increasing colour intensity from t_o to t₄). The scale for the microarray data is given at the left y-axis. Under ethanol biosynthesis conditions, cpcB– related transcripts decrease in abundance (black arrow). (B) Genome section containing the ribosomal protein operon (genes rpl3-4-23-2-rps19-rpl22-rps3-rpl16-29-rps17-rpl14-24-5-rps8-rpl6-18-rps5-rpl15). Under ethanol biosynthesis conditions, rps8–related transcripts increase as indicated by the arrow. WT, wild type.