Figure 6

Reduced accumulation of α phycocyanin in the producer (Pr1-3) in comparison to the control strain (Co1-3). (A) Soluble protein extract (3 μg) from three replicate cultures for each strain were separated using a 16% Tricine-SDS polyacrylamide gel containing urea (upper panel) and subjected to western blot analysis (lower panel) using a phycocyanin-specific antibody [16]. (B) Zinc fluorescence of covalently attached bilins visualized on a UV transilluminator in a 16% Tricine-SDS gel. Molecular masses inferred from protein marker VI, (M; AppliChem, Darmstadt, Germany) are shown on the right. The samples were prepared at day 14 of the experiment.