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Figure 3 | Biotechnology for Biofuels

Figure 3

From: New insights into enzymatic hydrolysis of heterogeneous cellulose by using carbohydrate-binding module 3 containing GFP and carbohydrate-binding module 17 containing CFP

Figure 3

Adsorptions of GC3 and CC17 proteins on 0.2 mg/mL of RAC in a 100 mM HEPES buffer (pH 7.5) containing 2 M NaCl at room temperature. (A) Single-component adsorption of GC3 and CC17. (B) Two-component adsorption of GC3 in the presence of 4.48 μmol/L CC17 and of CC17 in the presence of 1.54 μmol/L GC3. GC3 (■) and CC17 (). All curves were fit with the Langmuir isotherms. CC17, mono-cherry fluorescent protein linked to a family 17 carbohydrate-binding module; GC3, green fluorescent protein linked to a family 3 carbohydrate-binding module; RAC, regenerated amorphous cellulose.

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