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Table 1 Maximum adsorption capacities of the GC3 and CC17 on RAC and the determination of the beta value for CC17 that specifically binds to the amorphous cellulose surface

From: New insights into enzymatic hydrolysis of heterogeneous cellulose by using carbohydrate-binding module 3 containing GFP and carbohydrate-binding module 17 containing CFP

Protein Amax TCACa Alpha/beta value
  (μmol/g RAC) (m2/g RAC) (cellobiose lattice/protein)
GC3 8.64 ± 0.15 120 42.4
CC17 11.28 ± 0.26 120 32.5
  1. aTCAC = aCAC, because RAC is 100% amorphous cellulose [10]. aCAC, amorphous cellulose accessibility to cellulase; CC17, mono-cherry fluorescent protein linked to a family 17 carbohydrate-binding module; GC3, green fluorescent protein linked to a family 3 carbohydrate-binding module; RAC, regenerated amorphous cellulose; TCAC, total cellulose accessibility to cellulase.