Table 2 Metabolites differing in abundance between C. cellulolyticum WT and spo0A mutant cultures

2-Oxoisovalerate

0.32 ± 0.005

0.46 ± 0.03

0.56 ± 0.003

1.75 ± 0.16

18.7 ± 1.78

17.8 ± 0.89

0.08 ± 0.008

2-Oxobutyrate

0.20 ± 0.009

0.17 ± 0.03

0.19 ± 0.002

0.95 ± 0.24

1.63 ± 0.22

1.58 ± 0.19

0.04 ± 0.015

4-Methyl-2-oxovalerate

0.40 ± 0.05

2.43 ± 0.76

1.25 ± 0.24

0.04 ± 0.015

0.07 ± 0.03

0.06 ± 0.02

0.02 ± 0.01

2-Hydroxyglutarate

1.02 ± 0.27

16.51 ± 2.83

26.5 ± 4.12

0.76 ± 0.03

4.20 ± 0.41

3.83 ± 0.27

0.23 ± 0.01

2,3-Dihydroxyisovalerate

1.1 ± 0.27

0.35 ± 0.16

0.04

0.39 ± 0.04

0.15 ± 0.07

0.02 ± 0.006

ND

Malate

9.13 ± 1.39

20.2 ± 2.43

21.7 ± 1.63

3.70 ± 0.40

9.38 ± 0.32

7.59 ± 0.46

0.64 ± 0.05

2,3-Butanediol

0.74 ± 0.16

3.62 ± 0.03

5.13 ± 0.38

1.43 ± 0.24

12.71 ± 1.72

14.3 ± 2.24

0.05 ± 0.005

Glycerol-1phosphate

3.18 ± 0.05

3.36 ± 0.13

4.65 ± 0.72

3.29 ± 0.22

5.85 ± 0.62

6.72 ± 0.40

1.37 ± 0.07

Glucose-6phosphate

24.1 ± 3.76

59.3 ± 13.11

87.0 ± 8.80

9.12 ± 1.40

19.48 ± 6.67

35.3 ± 8.65

ND

Glucose-1phosphate

37.7 ± 8.35

17.3 ± 6.31

12.1 ± 0.18

48.4 ± 7.74

49.8 ± 3.17

38.9 ± 2.94

ND

11.10 min; 246 and 320 m/z

5.1 ± 0.28

13.1 ± 2.9

17.8 ± 2.8

0.61 ± 0.07

2.3 ± 0.50

1.43 ± 0.52

0.07 ± 0.02

7.52 min; 159 and 174 m/z

1.1 ± 0.007

0.98 ± 0.14

1.07 ± 0.09

2.43 ± 0.25

7.56 ± 0.51

7.0 ± 0.31

0.13 ± 0.03

9.58 min; 117, 259, 244, 288 and 303 m/z

0.2 ± 0.03

1.04 ± 0.01

0.52 ± 0.01

0.58 ± 0.11

4.26 ± 1.1

4.66 ± 0.99

0.14 ± 0.03

9.93 min; 331 and 359 m/z

11.7 ± 1.2

55.1 ± 11.6

29.6 ± 10.6

0.66 ± 0.18

2.59 ± 1.20

0.61 ± 0.05

0.07 ± 0.006

10.90 min; 450 m/z

7.94 ± 0.23

5.48 ± 1.19

3.83 ± 0.34

16.0 ± 1.55

21.6 ± 1.37

16.0 ± 1.55

0.08 ± 0.006