Skip to main content

Advertisement

Table 2 Metabolites differing in abundance between C. cellulolyticum WT and spo0A mutant cultures

From: Improvement of cellulose catabolism in Clostridium cellulolyticum by sporulation abolishment and carbon alleviation

  Wild-type growth phase spo0A growth phase  
Compound1 Log Stationary Late stationary Log Stationary Late stationary Sterile medium
2-Oxoisovalerate 0.32 ± 0.005 0.46 ± 0.03 0.56 ± 0.003 1.75 ± 0.16 18.7 ± 1.78 17.8 ± 0.89 0.08 ± 0.008
2-Oxobutyrate 0.20 ± 0.009 0.17 ± 0.03 0.19 ± 0.002 0.95 ± 0.24 1.63 ± 0.22 1.58 ± 0.19 0.04 ± 0.015
4-Methyl-2-oxovalerate 0.40 ± 0.05 2.43 ± 0.76 1.25 ± 0.24 0.04 ± 0.015 0.07 ± 0.03 0.06 ± 0.02 0.02 ± 0.01
2-Hydroxyglutarate 1.02 ± 0.27 16.51 ± 2.83 26.5 ± 4.12 0.76 ± 0.03 4.20 ± 0.41 3.83 ± 0.27 0.23 ± 0.01
2,3-Dihydroxyisovalerate 1.1 ± 0.27 0.35 ± 0.16 0.04 0.39 ± 0.04 0.15 ± 0.07 0.02 ± 0.006 ND
Malate 9.13 ± 1.39 20.2 ± 2.43 21.7 ± 1.63 3.70 ± 0.40 9.38 ± 0.32 7.59 ± 0.46 0.64 ± 0.05
2,3-Butanediol 0.74 ± 0.16 3.62 ± 0.03 5.13 ± 0.38 1.43 ± 0.24 12.71 ± 1.72 14.3 ± 2.24 0.05 ± 0.005
Glycerol-1phosphate 3.18 ± 0.05 3.36 ± 0.13 4.65 ± 0.72 3.29 ± 0.22 5.85 ± 0.62 6.72 ± 0.40 1.37 ± 0.07
Glucose-6phosphate 24.1 ± 3.76 59.3 ± 13.11 87.0 ± 8.80 9.12 ± 1.40 19.48 ± 6.67 35.3 ± 8.65 ND
Glucose-1phosphate 37.7 ± 8.35 17.3 ± 6.31 12.1 ± 0.18 48.4 ± 7.74 49.8 ± 3.17 38.9 ± 2.94 ND
11.10 min; 246 and 320 m/z 5.1 ± 0.28 13.1 ± 2.9 17.8 ± 2.8 0.61 ± 0.07 2.3 ± 0.50 1.43 ± 0.52 0.07 ± 0.02
7.52 min; 159 and 174 m/z 1.1 ± 0.007 0.98 ± 0.14 1.07 ± 0.09 2.43 ± 0.25 7.56 ± 0.51 7.0 ± 0.31 0.13 ± 0.03
9.58 min; 117, 259, 244, 288 and 303 m/z 0.2 ± 0.03 1.04 ± 0.01 0.52 ± 0.01 0.58 ± 0.11 4.26 ± 1.1 4.66 ± 0.99 0.14 ± 0.03
9.93 min; 331 and 359 m/z 11.7 ± 1.2 55.1 ± 11.6 29.6 ± 10.6 0.66 ± 0.18 2.59 ± 1.20 0.61 ± 0.05 0.07 ± 0.006
10.90 min; 450 m/z 7.94 ± 0.23 5.48 ± 1.19 3.83 ± 0.34 16.0 ± 1.55 21.6 ± 1.37 16.0 ± 1.55 0.08 ± 0.006
  1. 1Strains were grown in cellobiose medium. Metabolite concentrations were measured by gas chromatography–mass spectrometry analysis of the derivatized supernatants of duplicate (wild-type) or triplicate (mutant) cultures, as described in the Materials and methods section. The cultures were sampled at 20 hours after growth for log phase, 32 hours for stationary phase, and 48 hours for late stationary phase. Concentrations (μg/ml) are listed as the mean and standard deviation of measurements. Unidentified metabolites are represented by their chromatographic elution times and characteristic peak values. ND, not detected.