Table 1 Strains and plasmids used in this study
From: Hydrogen peroxide-independent production of α-alkenes by OleTJE P450 fatty acid decarboxylase
Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
E. coli strains | ||
BL21(DE3) | F-ompT gal dcm lon hsdSB (rB- mB-) λ(DE3) | Novagen |
DH5α | F- ξ80lacZ ΔM15 Δ (ΔlacZYA-argF) U169 recA 1 endA 1 hsdR 17 (rk-, mk+) phoA supE 44 thi- 1 gyrA96 relA1 λ- | Invitrogen |
XL100 | BL21: ΔfadDa | [43] |
YL1 | BL21(DE3) with pET28b-oleT JE | This study |
YL2 | BL21(DE3) with pET28b-oleT JE -RhFRED | This study |
YL3 | BL21(DE3) with pACYCDuet-fdR | This study |
YL4 | BL21(DE3) with pCDEDuet-fld | This study |
YL5 | XL100 with pET28b-oleT JE | This study |
YL6 | XL100 with pET28b-oleT JE -RhFRED | This study |
YL7 | XL100 with pET28b-oleT JE , pMSD8b, pMSD15c | This study |
YL8 | XL100 with pET28b-oleT JE -RhFRED, pMSD8, pMSD15 | This study |
Jeotgalicoccus sp. ATCC 8456 | Wild type | ATCC |
Plasmids | ||
pET28b | Kmr, T7 promoter, pBR322 origin | Novagen |
pET28b-oleT JE | Kmr, pET28b derivative containing oleT JE gene | This study |
pET28b-oleT JE -RhFRED | Kmr, pET28b derivative containing oleT JE and RhFRED gene | This study |
pACYCDuet-1 | Cmr, T7 promoter, P15A origin | Novagen |
pACYCDuet-fdR | Cmr, pACYCDuet-1 derivative containing fdR gene | This study |
pCDFDuet-1 | Strr, T7 promoter, CloDF13 origin | Novagen |
pCDEDuet-fld | Strr, pCDFDuet-1 derivative containing fld gene | This study |
pMSD8 | Ampr, P T7 : accB, C, D, A, pSC101 origin | [43] |
pMSD15 | Cmr, P BAD : tesA, p15a origin | [43] |