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Table 1 Strains and plasmids used in this study

From: Hydrogen peroxide-independent production of α-alkenes by OleTJE P450 fatty acid decarboxylase

Strain or plasmid Relevant characteristics Source or reference
E. coli strains   
BL21(DE3) F-ompT gal dcm lon hsdSB (rB- mB-) λ(DE3) Novagen
DH5α F- ξ80lacZ ΔM15 Δ (ΔlacZYA-argF) U169 recA 1 endA 1 hsdR 17 (rk-, mk+) phoA supE 44 thi- 1 gyrA96 relA1 λ- Invitrogen
XL100 BL21: ΔfadDa [43]
YL1 BL21(DE3) with pET28b-oleT JE This study
YL2 BL21(DE3) with pET28b-oleT JE -RhFRED This study
YL3 BL21(DE3) with pACYCDuet-fdR This study
YL4 BL21(DE3) with pCDEDuet-fld This study
YL5 XL100 with pET28b-oleT JE This study
YL6 XL100 with pET28b-oleT JE -RhFRED This study
YL7 XL100 with pET28b-oleT JE , pMSD8b, pMSD15c This study
YL8 XL100 with pET28b-oleT JE -RhFRED, pMSD8, pMSD15 This study
Jeotgalicoccus sp. ATCC 8456 Wild type ATCC
Plasmids   
pET28b Kmr, T7 promoter, pBR322 origin Novagen
pET28b-oleT JE Kmr, pET28b derivative containing oleT JE gene This study
pET28b-oleT JE -RhFRED Kmr, pET28b derivative containing oleT JE and RhFRED gene This study
pACYCDuet-1 Cmr, T7 promoter, P15A origin Novagen
pACYCDuet-fdR Cmr, pACYCDuet-1 derivative containing fdR gene This study
pCDFDuet-1 Strr, T7 promoter, CloDF13 origin Novagen
pCDEDuet-fld Strr, pCDFDuet-1 derivative containing fld gene This study
pMSD8 Ampr, P T7 : accB, C, D, A, pSC101 origin [43]
pMSD15 Cmr, P BAD : tesA, p15a origin [43]
  1. aThe fadD gene encodes the acyl-CoA synthetase, which is the first enzyme involved in fatty acid degradation. bpMSD8: the expression vector for E. coli acyl-CoA carboxylase. cpMSD15: the plasmid used for overexpression of the E. coli thioesterase gene tesA. Amp, ampicillin; Cm, chloramphenicol; Km, kanamycin; Str, streptomycin.