Figure 4

Temperature optimum and stability of the recombinant xylanases. (A) The temperature optima were measured under the standard assay conditions, but temperatures ranged from 35 to 60°C for reAuXyn11A as well as from 45 to 80°C for reAEXynM and its recombinant mutants. (B) To estimate the temperature stabilities, reAuXyn11A and reAEXynMC5T were incubated from 35 to 60°C and other recombinant xylanases from 45 to 80°C, respectively, for 1.0 hour. ■, reAuXyn11A; ▲, reAEXynM; □, reAEXynMC5T; △, reAEXynMP9S; ○, reAEXynMH14N.