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Table 1 Fatty acid content of wild-type and transgenic Synechocystis sp. PCC6803 a–d

From: Transgenic expression of delta-6 and delta-15 fatty acid desaturases enhances omega-3 polyunsaturated fatty acid accumulation in Synechocystissp. PCC6803

Strain T, °C TFA, mg/g FA content, %
    C18:1 C18:2 C18:3n-6 C18:3n-3 C18:4 Others
Wild type 30 75.201 7.07 ± 0.9 13.59 ± 1.0 16.26 ± 1.2 1.45 ± 0.2 1.20 ± 0.3 60.44 ± 4.3
20 60.640 3.94 ± 0.2 16.75 ± 0.6 14.72 ± 1.3 2.53 ± 0.3 1.54 ± 0.2 60.53 ± 3.6
pSDSy15 30 50.755 2.73 ± 0.3 2.80 ± 0.5 0.30 ± 0.1 17.52 ± 2.3 9.11 ± 1.3 67.54 ± 7.3
20 60.760 3.27 ± 0.7 1.94 ± 0.3 0.23 ± 0.1 23.05 ± 2.3 10.77 ± 1.6 60.73 ± 5.7
pSDGf1215 30 67.085 3.17 ± 0.3 15.56 ± 1.3 13.79 ± 2.0 1.78 ± 0.3 1.20 ± 0.2 64.50 ± 5.5
20 66.990 5.73 ± 1.3 17.67 ± 1.0 12.71 ± 0.8 1.63 ± 0.1 0.96 ± 0.2 61.29 ± 8.3
pSDSy15Sy6 30 63.071 4.10 ± 0.8 2.57 ± 0.6 0.21 ± 0.1 23.64 ± 3.4 7.76 ± 0.7 61.72 ± 8.1
20 57.130 2.28 ± 0.2 1.30 ± 0.3 0.19 ± 0.1 16.35 ± 1.9 13.12 ± 1.3 66.77 ± 6.5
pSDGf1215M6 30 68.803 3.11 ± 0.4 17.21 ± 2.3 14.45 ± 1.6 2.05 ± 0.2 1.18 ± 0.1 61.98 ± 5.3
20 75.657 1.20 ± 0.1 10.65 ± 2.3 17.16 ± 1.6 3.02 ± 0.5 1.70 ± 0.1 66.27 ± 5.9
pSDSy15Ma6 30 61.480 3.45 ± 0.2 0.70 ± 0.1 0.21 ± 0.1 17.79 ± 2.3 11.10 ± 1.5 66.76 ± 3.9
20 35.190 2.03 ± 0.2 1.22 ± 0.3 0.14 ± 0.1 14.83 ± 2.9 12.36 ± 1.5 69.42 ± 5.3
pSDGf1215Sy6 30 59.192 2.80 ± 0.2 15.98 ± 0.6 13.95 ± 0.7 2.40 ± 0.4 1.57 ± 0.1 63.32 ± 6.0
  20 53.651 6.75 ± 1.2 17.17 ± 2.0 10.81 ± 0.9 2.11 ± 0.6 0.89 ± 0.1 62.27 ± 8.7
  1. FA, fatty acid; T, Temperature; TFA, Total fatty acid.
  2. aValues are means of triplicate experiments.
  3. bCells were grown under a light intensity of 40 μmol photon/m2/s for 10 d in BG-11 medium.
  4. cThe membrane lipids were extracted from wild-type and genetically engineered Synechocystis sp. PCC6803.
  5. dThe enzymes overexpressed are indicated in parentheses (Sy15: Δ15 FA desaturase from Synechocystis sp. PCC6803; Sy6: Δ6 FA desaturase from Synechocystis sp. PCC6803; Gf1215: bifunctional Δ12/Δ15 FA desaturase from Gibberella fujikuroi; Ma6: Δ6 FA desaturase from Mortierella alpina).