Skip to main content

Table 1 Fatty acid content of wild-type and transgenic Synechocystis sp. PCC6803 a–d

From: Transgenic expression of delta-6 and delta-15 fatty acid desaturases enhances omega-3 polyunsaturated fatty acid accumulation in Synechocystissp. PCC6803

Strain

T, °C

TFA, mg/g

FA content, %

   

C18:1

C18:2

C18:3n-6

C18:3n-3

C18:4

Others

Wild type

30

75.201

7.07 ± 0.9

13.59 ± 1.0

16.26 ± 1.2

1.45 ± 0.2

1.20 ± 0.3

60.44 ± 4.3

20

60.640

3.94 ± 0.2

16.75 ± 0.6

14.72 ± 1.3

2.53 ± 0.3

1.54 ± 0.2

60.53 ± 3.6

pSDSy15

30

50.755

2.73 ± 0.3

2.80 ± 0.5

0.30 ± 0.1

17.52 ± 2.3

9.11 ± 1.3

67.54 ± 7.3

20

60.760

3.27 ± 0.7

1.94 ± 0.3

0.23 ± 0.1

23.05 ± 2.3

10.77 ± 1.6

60.73 ± 5.7

pSDGf1215

30

67.085

3.17 ± 0.3

15.56 ± 1.3

13.79 ± 2.0

1.78 ± 0.3

1.20 ± 0.2

64.50 ± 5.5

20

66.990

5.73 ± 1.3

17.67 ± 1.0

12.71 ± 0.8

1.63 ± 0.1

0.96 ± 0.2

61.29 ± 8.3

pSDSy15Sy6

30

63.071

4.10 ± 0.8

2.57 ± 0.6

0.21 ± 0.1

23.64 ± 3.4

7.76 ± 0.7

61.72 ± 8.1

20

57.130

2.28 ± 0.2

1.30 ± 0.3

0.19 ± 0.1

16.35 ± 1.9

13.12 ± 1.3

66.77 ± 6.5

pSDGf1215M6

30

68.803

3.11 ± 0.4

17.21 ± 2.3

14.45 ± 1.6

2.05 ± 0.2

1.18 ± 0.1

61.98 ± 5.3

20

75.657

1.20 ± 0.1

10.65 ± 2.3

17.16 ± 1.6

3.02 ± 0.5

1.70 ± 0.1

66.27 ± 5.9

pSDSy15Ma6

30

61.480

3.45 ± 0.2

0.70 ± 0.1

0.21 ± 0.1

17.79 ± 2.3

11.10 ± 1.5

66.76 ± 3.9

20

35.190

2.03 ± 0.2

1.22 ± 0.3

0.14 ± 0.1

14.83 ± 2.9

12.36 ± 1.5

69.42 ± 5.3

pSDGf1215Sy6

30

59.192

2.80 ± 0.2

15.98 ± 0.6

13.95 ± 0.7

2.40 ± 0.4

1.57 ± 0.1

63.32 ± 6.0

 

20

53.651

6.75 ± 1.2

17.17 ± 2.0

10.81 ± 0.9

2.11 ± 0.6

0.89 ± 0.1

62.27 ± 8.7

  1. FA, fatty acid; T, Temperature; TFA, Total fatty acid.
  2. aValues are means of triplicate experiments.
  3. bCells were grown under a light intensity of 40 μmol photon/m2/s for 10 d in BG-11 medium.
  4. cThe membrane lipids were extracted from wild-type and genetically engineered Synechocystis sp. PCC6803.
  5. dThe enzymes overexpressed are indicated in parentheses (Sy15: Δ15 FA desaturase from Synechocystis sp. PCC6803; Sy6: Δ6 FA desaturase from Synechocystis sp. PCC6803; Gf1215: bifunctional Δ12/Δ15 FA desaturase from Gibberella fujikuroi; Ma6: Δ6 FA desaturase from Mortierella alpina).