Figure 1

sdp1-5 mutant accumulates higher triacylglycerol (TAG) levels than wild type (Columbia-0; Col-0) in mature seeds. (A) Scanning electron microscopy (SEM) showing the seed surface structure of WT (Col-0) and sdp1-5; (B) comparative dry seed weight of WT (Col-0) and sdp1-5; (C) total amount of FAs per seed of WT (Col-0) and sdp1-5; (D) relative FAs of dried seeds of WT (Col-0) and sdp1-5; (E) FA profile of WT (Col-0) and sdp1-5 seeds. (F) Seed eicosenoic acid (20:1) content; (G) thin layer chromatography (TLC) separation of neutral lipid fractions from WT (Col-0) and three lines of sdp1-5 null mutant; 300 μg of neutral lipids were fractionated by TLC on silica gel plates. DAG, diacylglycerol, FA, fatty acid; FFA, free fatty acid (oleic acid), Mix, mixture of TAG and FFA; SE, sterol ester; TAG, Triacylglycerol (triolein). (H) Profiling of relative amounts of FFA and TAG by GC/MS. The absolute amount was calculated using C15:0 as an internal control by comparing their peak areas. *P?<?0.05 or **P?<?0.01 versus WT (Col-0). Each experiment was performed with 100 seeds per line with 5 biological replicates. Error bar shows standard deviation (SD) (n?=?5). DW, dry weight.