Figure 2
sdp1-5 mutant seeds produce more oil bodies. (A) Transmission electron microscopy (TEM) analysis of oil body distribution in wild type (Columbia-0; Col-0) and sdp1-5 mature seeds. Scale bar?=?5 μm. CW, cell wall; OB, oil body; PSV, protein storage vacuole. (B) Number of oil bodies per cell in mature seeds of WT (Col-0) and sdp1-5. A cell has an average of 196 μm2 cross-sectional area. Values are mean?±?SD; n?=?5. (C) Expression levels of AtOLE1 (At4g25140), AtOLE2 (At5g40420), and AtOLE3 (At5g51210) in early developing stages of seeds (3 to 5 DAP) from WT (Col-0) and sdp1-5. Actin1 (At2g37620) was used as an internal control. *P?<?0.05, **P?<?0.01, or ***P?<?0.001 versus WT (Col-0). There were five biological replicates. Values are mean?±?SD; n?=?5. DAP, days after pollination.