Figure 1

Gene expression analysis. (A) Western blot showing transient expression of faeB proteins in tobacco leaves. L1, protein ladder (catalogue number 161-0374; Bio-Rad, Hercules, CA, USA); L2, uninoculated leaf proteins; L3, proteins of tobacco leaves infected with LBA4404 (no construct); L4, proteins of tobacco leaves infected with agro harboring pEACH 5,103; L5, proteins of tobacco leaves infected with agro harboring faeB-apoplast; L6, proteins of tobacco leaves infected with agro harboring faeB-chloroplast; L7, proteins of tobacco leaves infected with agro harboring faeB-ER; and L8, proteins of tobacco leaves infected with agro harboring faeB-vacuole. (B) Transient expression of GUS in alfalfa leaves. tCUP:GUS was stably transformed into alfalfa plants (obtained from Dr Lining Tian, Agriculture and Agri-Food Canada London, ON, Canada), served as a positive control. ER, endoplasmic reticulum; GUS, β-glucuronidase.