Figure 2From: Expression of a fungal ferulic acid esterase in alfalfa modifies cell wall digestibilityFAEB activity assay, PCR validation of faeB and immunocytochemical localization of FAEB. (A) FAEB activity as indicated by the change in absorbance over 60Â min as a result of the hydrolysis of ethyl ferulate. Data represents three absorbance readings from the same extract from a single plant. Error bars indicate relative standard error. Apoplast (A), transgenic line 3A; control, wild type plant; endoplasmic reticulum (ER), transgenic line 28ER and vacuole (V), transgenic line 2Â V. (B) PCR validation of faeB gene in transgenic lines. Apoplast-expressing sample: lane 1, nptII (apoplast); lane 2, faeB-apoplast and lane 3, wild type negative control for faeB-apoplast. ER-expressing sample: lane 4, nptII (ER); lane 5, faeB-ER and lane 6, wild type negative control for faeB-ER. Vacuole-expressing sample: lane 7, nptII (vacuole); lane 8, faeB-vacuole and lane 9, wild type negative control for faeB-vacuole. (C) Immunocytochemical localization to confirm recombinant protein expression in: (a) apoplast; (b) endoplasmic reticulum; (c) vacuole and (d) non-transgenic control in alfalfa leaves. A, apoplast; ER, endoplasmic reticulum; FAEB, type B ferulic acid esterase; V, vacuole.Back to article page