Lignin content and sugar compositional analysis. (A) Acetyl bromide soluble lignin content. Bars indicate standard error of mean (n = 3). *Differs to control at P 0.05. (B) Sugar composition of control and transgenic alfalfa. Sugars were quantified as alditol acetate derivatives by GC-MS. Error bars indicate standard deviation (n = 3) of technical replicates from ground samples pooled from 50 to 60 whole plants per genotype. Alfalfa lines indicate expression of faeB in endoplasmic reticulum (ER), 24ER and 28ER; apoplast (A), 43A, 41A and 1A; and vacuole (V), 61 V, 15 V and 2 V. A, apoplast; C, wild type control; ER, endoplasmic reticulum; GC-MS, gas chromatography–mass spectrometry; V, vacuole.