Comparison of single-molecule sequencing and hybrid approaches for finishing the genome of Clostridium autoethanogenum and analysis of CRISPR systems in industrial relevant Clostridia

Table 2 Assembly statistics for strain DSM 10061

	Contigs (number)	Largest contig (bp)	Contig N50 (bp)	Genome size (Mb)	Scaffolds	Largest scaffold (bp)	Scaffold N50 (bp)	Assembler
454/Ion Torrent ^*	100	436,795	115,901	4.32				Newbler 2.6
Illumina only	57	460,940	255,482	4.3	53	769,812	328,660	Velvet 1.2
454 only	32	134,546	330,116	4.3	13	1,137,876	898,466	Newbler 2.8
Illumina/ 454 Hybrid	22	1,137,625	687,076	4.3	13	1,137,625	899,926	Newbler 2.8
PacBio	1	4,352,205	4,352,267	4.3	1	4,352,267	4,352,267	SMRT 2.0

^*Previously published as a 4.5-Mb draft genome [26], but present [GenBank: ASZX00000000.1] as 4,323,309 bp.