Skip to main content

Advertisement

Table 2 Identification by MALDI-MS and ESI-LC MS/MS of proteins secreted by T. reesei Rut C-30 grown in CMM and LCM

From: Cost-effective lignocellulolytic enzyme production by Trichoderma reesei on a cane molasses medium

Number ProtIDa Protein name Precursor mass (kDa) pIb Mascot scorec Peptides matched Sequence coverage (%)
1d 123989 Cellobiohydrolase I (Cel7A) 55.4 4.6 73 5 13
2d 123989 Cellobiohydrolase I (Cel7A) 55.4 4.5 73 5 13
3d 123989 Cellobiohydrolase I (Cel7A) 55.4 4.5 73 5 13
4d 123989 Cellobiohydrolase I (Cel7A) 55.4 4.5 73 5 13
5d 123989 Cellobiohydrolase I (Cel7A) 55.4 4.5 73 5 13
6e 123989 Cellobiohydrolase I (Cel7A) 54.1 4.4 598 21 24.9
7e 123989 Cellobiohydrolase I (Cel7A) 54.1 4.5 201 8 16.7
8d 72567 Cellobiohydrolase II (Cel6A) 50.3 4.8 107 10 27
9d 72567 Cellobiohydrolase II (Cel6A) 50.3 4.9 100 9 27
10d 72567 Cellobiohydrolase II (Cel6A) 50.3 5.0 83 8 26
11d 72567 Cellobiohydrolase II (Cel6A) 49.6 5.1 133 5 13.4
12e 72567 Cellobiohydrolase II (Cel6A) 35.1 4.7 471 22 21.4
13e 72567 Cellobiohydrolase II (Cel6A) 49.6 5.5 94 5 6.6
14e 72567 Cellobiohydrolase II (Cel6A) 39.2 4.6 135 8 9.2
15d 120312 Endoglucanase II (Cel5A) 48.7 5.8 56 4 24
16e 120312 Endoglucanase II (Cel5A) 44.1 4.5 340 23 30.4
17e 120312 Endoglucanase II (Cel5A) 44.1 4.7 219 5 18.4
18e 122081 Endoglucanase I (Cel7B) 48.2 4.6 106 4 9.2
19d 123232 Endoglucanase III (Cel12A) 25.1 4.5 83 8 32
20d 76672 β-Glucosidase I (BGLI) 78.7 6.4 196 20 31.2
21d 76672 β-Glucosidase I (BGLI) 78.7 6.5 106 17 23.6
22d 123283 Arabinofuranosidase (ABFI) 40.2 5.5 113 11 25.1
23d 49081 Xyloglucanase (Cel74A) 87.3 5.2 184 15 29
24d 49081 Xyloglucanase (Cel74A) 87.3 5.4 123 11 24.7
25d 121127 β-Xylosidase (BXLI) 87.5 5.5 188 21 41
26d 121127 β-Xylosidase (BXLI) 87.5 5.6 132 15 24.1
27d 121127 β-Xylosidase (BXLI) 87.5 5.7 101 14 26.3
28d 121127 β-Xylosidase (BXLI) 87.5 5.8 155 17 28.6
29e 74223 Xylanase I (XYNI) 24.6 4.7 142 3 9.4
30d 111849 Xylanase IV (XYNIV) 53.4 5.9 139 10 24
31e 73638 Cellulose-binding protein (CIPI) 32.9 4.8 317 13 20.3
32d 123940 Cellulose-binding protein (CIPII) 49.0 6.3 107 13 29
33d 56996 Mannanase I (MANI) 40.1 4.8 97 8 19.2
34d 73643 Endoglucanase IV (Cel61A) 40.2 4.9 86 2 6.1
35d 121418 Lipolytic enzyme (GDSL) 39.3 5.7 113 11 34
  1. aFrom The Genome Portal of the Department of Energy Joint Genome Institute (JGI) for T. reesei; bpI determined by 2-DE; canalyzed by dMALDI-MS or eESI-LC MS/MS. 2-DE, two-dimensional gel electrophoresis; CMM, cane molasses medium; ESI-LC MS/MS, electrospray ionization liquid chromatography tandem mass spectrometry; LCM, lactose-based conventional medium; MALDI-MS, matrix-assisted laser desorption/ionization mass spectrometry.