Table 2 Identification by MALDI-MS and ESI-LC MS/MS of proteins secreted by T. reesei Rut C-30 grown in CMM and LCM
From: Cost-effective lignocellulolytic enzyme production by Trichoderma reesei on a cane molasses medium
Number | ProtIDa | Protein name | Precursor mass (kDa) | pIb | Mascot scorec | Peptides matched | Sequence coverage (%) |
|---|---|---|---|---|---|---|---|
1d | 123989 | Cellobiohydrolase I (Cel7A) | 55.4 | 4.6 | 73 | 5 | 13 |
2d | 123989 | Cellobiohydrolase I (Cel7A) | 55.4 | 4.5 | 73 | 5 | 13 |
3d | 123989 | Cellobiohydrolase I (Cel7A) | 55.4 | 4.5 | 73 | 5 | 13 |
4d | 123989 | Cellobiohydrolase I (Cel7A) | 55.4 | 4.5 | 73 | 5 | 13 |
5d | 123989 | Cellobiohydrolase I (Cel7A) | 55.4 | 4.5 | 73 | 5 | 13 |
6e | 123989 | Cellobiohydrolase I (Cel7A) | 54.1 | 4.4 | 598 | 21 | 24.9 |
7e | 123989 | Cellobiohydrolase I (Cel7A) | 54.1 | 4.5 | 201 | 8 | 16.7 |
8d | 72567 | Cellobiohydrolase II (Cel6A) | 50.3 | 4.8 | 107 | 10 | 27 |
9d | 72567 | Cellobiohydrolase II (Cel6A) | 50.3 | 4.9 | 100 | 9 | 27 |
10d | 72567 | Cellobiohydrolase II (Cel6A) | 50.3 | 5.0 | 83 | 8 | 26 |
11d | 72567 | Cellobiohydrolase II (Cel6A) | 49.6 | 5.1 | 133 | 5 | 13.4 |
12e | 72567 | Cellobiohydrolase II (Cel6A) | 35.1 | 4.7 | 471 | 22 | 21.4 |
13e | 72567 | Cellobiohydrolase II (Cel6A) | 49.6 | 5.5 | 94 | 5 | 6.6 |
14e | 72567 | Cellobiohydrolase II (Cel6A) | 39.2 | 4.6 | 135 | 8 | 9.2 |
15d | 120312 | Endoglucanase II (Cel5A) | 48.7 | 5.8 | 56 | 4 | 24 |
16e | 120312 | Endoglucanase II (Cel5A) | 44.1 | 4.5 | 340 | 23 | 30.4 |
17e | 120312 | Endoglucanase II (Cel5A) | 44.1 | 4.7 | 219 | 5 | 18.4 |
18e | 122081 | Endoglucanase I (Cel7B) | 48.2 | 4.6 | 106 | 4 | 9.2 |
19d | 123232 | Endoglucanase III (Cel12A) | 25.1 | 4.5 | 83 | 8 | 32 |
20d | 76672 | β-Glucosidase I (BGLI) | 78.7 | 6.4 | 196 | 20 | 31.2 |
21d | 76672 | β-Glucosidase I (BGLI) | 78.7 | 6.5 | 106 | 17 | 23.6 |
22d | 123283 | Arabinofuranosidase (ABFI) | 40.2 | 5.5 | 113 | 11 | 25.1 |
23d | 49081 | Xyloglucanase (Cel74A) | 87.3 | 5.2 | 184 | 15 | 29 |
24d | 49081 | Xyloglucanase (Cel74A) | 87.3 | 5.4 | 123 | 11 | 24.7 |
25d | 121127 | β-Xylosidase (BXLI) | 87.5 | 5.5 | 188 | 21 | 41 |
26d | 121127 | β-Xylosidase (BXLI) | 87.5 | 5.6 | 132 | 15 | 24.1 |
27d | 121127 | β-Xylosidase (BXLI) | 87.5 | 5.7 | 101 | 14 | 26.3 |
28d | 121127 | β-Xylosidase (BXLI) | 87.5 | 5.8 | 155 | 17 | 28.6 |
29e | 74223 | Xylanase I (XYNI) | 24.6 | 4.7 | 142 | 3 | 9.4 |
30d | 111849 | Xylanase IV (XYNIV) | 53.4 | 5.9 | 139 | 10 | 24 |
31e | 73638 | Cellulose-binding protein (CIPI) | 32.9 | 4.8 | 317 | 13 | 20.3 |
32d | 123940 | Cellulose-binding protein (CIPII) | 49.0 | 6.3 | 107 | 13 | 29 |
33d | 56996 | Mannanase I (MANI) | 40.1 | 4.8 | 97 | 8 | 19.2 |
34d | 73643 | Endoglucanase IV (Cel61A) | 40.2 | 4.9 | 86 | 2 | 6.1 |
35d | 121418 | Lipolytic enzyme (GDSL) | 39.3 | 5.7 | 113 | 11 | 34 |