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Table 2 Comparison of K. pneumoniae parent strain (KG1) and the Δ ldhA using several carbon sources in a 12-h flask cultivation, the values represent mean ± SD

From: Effect of the inactivation of lactate dehydrogenase, ethanol dehydrogenase, and phosphotransacetylase on 2,3-butanediol production in Klebsiella pneumoniae strain

Strain Carbon source (50 g/L) Consumed carbon source (g/L) 2,3-Butanediol (g/L) L-Lactate (g/L) D-Lactate (g/L) Acetate (g/L) Acetoin (g/L) Ethanol (g/L) Biomass (g/L) 2,3-Butanediol yield (g/g) RH RC
KG1 Glucose 44.41 ± 0.45 13.82 ± 0.05 2.05 ± 0.03 3.34 ± 0.15 0.70 ± 0.04 0.65 ± 0.02 3.24 ± 0.21 4.63 ± 0.14 0.31 0.82 0.98
Lactose 24.04 ± 0.23 5.16 ± 0.03 2.05 ± 0.02 0.53 ± 0.03 1.35 ± 0.10 0.35 ± 0.03 1.29 ± 0.05 2.42 ± 0.08 0.21 0.66 1.57
Fructose 38.11 ± 0.32 13.26 ± 0.11 2.64 ± 0.04 1.53 ± 0.06 0.81 ± 0.06 0.54 ± 0.06 3.68 ± 0.12 4.02 ± 0.07 0.35 0.97 1.13
Galactose 31.52 ± 0.28 10.45 ± 0.08 1.92 ± 0.02 3.31 ± 0.18 0.80 ± 0.07 0.67 ± 0.04 3.30 ± 0.11 3.49 ± 0.11 0.33 1.01 1.28
Maltose 41.63 ± 0.35 15.05 ± 0.10 3.19 ± 0.03 2.58 ± 0.21 0.38 ± 0.02 4.53 ± 0.23 3.54 ± 0.05 0.36 1.24 1.30
ΔldhA Glucose 44.74 ± 0.21 15.87 ± 0.08 2.38 ± 0.03 0.05 ± 0.01 0.83 ± 0.04 1.75 ± 0.10 5.42 ± 0.32 4.84 ± 0.05 0.35 1.04 1.13
Lactose 18.24 ± 0.15 2.97 ± 0.05 1.66 ± 0.02 0.44 ± 0.05 2.16 ± 0.04 0.19 ± 0.03 1.29 ± 0.16 1.57 ± 0.03 0.16 0.54 1.34
Fructose 49.52 ± 0.22 16.97 ± 0.24 3.04 ± 0.04 0.21 ± 0.06 0.30 ± 0.05 0.67 ± 0.02 4.92 ± 0.18 4.49 ± 0.08 0.34 1.11 0.99
Galactose 47.04 ± 0.28 14.33 ± 0.15 2.82 ± 0.02 0.12 ± 0.02 0.81 ± 0.03 2.11 ± 0.11 5.55 ± 0.22 4.49 ± 0.04 0.30 1.13 1.09
  Maltose 49.59 ± 0.35 18.75 ± 0.11 3.95 ± 0.07 0.08 ± 0.02 0.27 ± 0.05 1.37 ± 0.05 6.01 ± 0.24 5.06 ± 0.15 0.38 1.18 1.22