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Table 3 Comparison of K. pneumoniae parent strain (KG1), Δ adhE and the Δ pta using several carbon sources in a 36-h flask cultivation, the values represent mean ± SD

From: Effect of the inactivation of lactate dehydrogenase, ethanol dehydrogenase, and phosphotransacetylase on 2,3-butanediol production in Klebsiella pneumoniae strain

Strain

Carbon source (50 g/L)

Consumed carbon source (g/L)

2,3-Butanediol (g/L)

Lactate (g/L)

Acetate (g/L)

Acetoin (g/L)

Ethanol (g/L)

Biomass (g/L)

2,3-Butanediol yield (g/g)

KG1

Glucose

49.29 ± 0.65

16.76 ± 0.22

3.62 ± 0.21

4.52 ± 0.45

1.73 ± 0.09

2.67 ± 0.16

4.80 ± 0.32

0.34

Lactose

49.39 ± 0.59

16.28 ± 0.13

2.95 ± 0.15

2.98 ± 0.28

3.26 ± 0.11

4.16 ± 0.32

2.80 ± 0.43

0.33

Fructose

44.84 ± 0.61

14.41 ± 0.16

2.27 ± 0.12

2.85 ± 0.33

2.46 ± 0.13

3.22 ± 0.25

4.06 ± 0.52

0.32

Galactose

34.89 ± 0.48

10.27 ± 0.11

3.33 ± 0.15

2.83 ± 0.32

1.80 ± 0.10

2.64 ± 0.28

3.89 ± 0.18

0.29

Maltose

49.20 ± 0.51

17.30 ± 0.21

2.03 ± 0.14

2.79 ± 0.16

3.56 ± 0.12

4.45 ± 0.45

3.87 ± 0.21

0.35

ΔadhE

Glucose

49.27 ± 0.42

23.65 ± 0.25

1.35 ± 0.09

2.05 ± 0.12

0.60 ± 0.04

0.52 ± 0.04

3.95 ± 0.52

0.48

Lactose

17.76 ± 0.25

8.76 ± 0.12

1.61 ± 0.11

3.85 ± 0.42

2.01 ± 0.08

0.36 ± 0.06

1.76 ± 0.35

0.49

Fructose

49.47 ± 0.52

21.40 ± 0.24

1.48 ± 0.13

1.58 ± 0.12

0.83 ± 0.06

0.94 ± 0.02

3.25 ± 0.28

0.43

Galactose

36.35 ± 0.42

14.05 ± 0.42

0.73 ± 0.07

1.96 ± 0.09

3.70 ± 0.21

0.65 ± 0.03

2.99 ± 0.42

0.39

Maltose

49.49 ± 0.47

18.18 ± 0.23

2.96 ± 0.10

1.27 ± 0.08

4.58 ± 0.24

0.91 ± 0.08

4.27 ± 0.25

0.36

Δpta

Glucose

49.27 ± 0.54

20.20 ± 0.62

0.77 ± 0.09

3.15 ± 0.11

2.31 ± 0.15

1.69 ± 0.32

3.40 ± 0.52

0.41

Lactose

10.10 ± 0.25

2.98 ± 0.11

0.96 ± 0.07

2.01 ± 0.10

_

0.30 ± 0.02

1.01 ± 0.46

0.30

Fructose

49.51 ± 0.55

18.36 ± 0.32

1.01 ± 0.10

2.20 ± 0.09

2.81 ± 0.11

1.87 ± 0.14

2.96 ± 0.26

0.37

Galactose

39.49 ± 0.23

11.88 ± 0.12

0.19 ± 0.06

1.98 ± 0.12

1.56 ± 0.14

1.29 ± 0.21

3.27 ± 0.43

0.30

 

Maltose

40.28 ± 0.54

14.57 ± 0.24

4.68 ± 0.08

2.50 ± 0.14

1.91 ± 0.12

3.26 ± 0.33

3.74 ± 0.44

0.36