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Table 4 Strains and plasmids used in this work

From: Effect of the inactivation of lactate dehydrogenase, ethanol dehydrogenase, and phosphotransacetylase on 2,3-butanediol production in Klebsiella pneumoniae strain

Strain, plasmid Genotype, properties Source or reference
Strains
K. pneumoniae
KG1 parent strain (KG1) [19]
ΔldhA A lactate-deficient mutant of KG1 This work
ΔadhE An alcohol dehydrogenase-deficient mutant of KG1 This work
Δpta An phosphate acetyltransferase gene-deficient mutant of KG1 This work
ΔldhA ΔadhE A lactate and alcohol dehydrogenase, both deficient mutants of KG1 This work
E. coli   
DH5α Φ80 lacZ ΔM15 ΔlacU169 recA1 endA1 hsdR17 supE44 thi-1 gyrA relA1 TaKaRa
S17-1 λpir TpR SmR recA thi-1 pro hsdR-M+RP4: 2-Tc:Mu: Km Tn7 λpir [31]
Plasmids
pRE112 Suicide vector CmR SacB oriT oriV [31]
p-ldh A’ CmR, pRE112 derivative, where a 625-bp DNA fragment containing the ldhA’ was inserted This work
p-adh E’ CmR, pRE112 derivative, where a 872-bp DNA fragment containing the adhE’ was inserted This work
p-pta CmR, pRE112 derivative, where a 901-bp DNA fragment containing the pta’ was inserted This work