Effect of the inactivation of lactate dehydrogenase, ethanol dehydrogenase, and phosphotransacetylase on 2,3-butanediol production in Klebsiella pneumoniae strain

Table 4 Strains and plasmids used in this work

Strain, plasmid	Genotype, properties	Source or reference
Strains
K. pneumoniae
KG1	parent strain (KG1)	[19]
ΔldhA	A lactate-deficient mutant of KG1	This work
ΔadhE	An alcohol dehydrogenase-deficient mutant of KG1	This work
Δpta	An phosphate acetyltransferase gene-deficient mutant of KG1	This work
ΔldhA ΔadhE	A lactate and alcohol dehydrogenase, both deficient mutants of KG1	This work
E. coli
DH5α	Φ80 lacZ ΔM15 ΔlacU169 recA1 endA1 hsdR17 supE44 thi-1 gyrA relA1	TaKaRa
S17-1 λpir	TpR SmR recA thi-1 pro hsdR-M⁺RP4: 2-Tc:Mu: Km Tn7 λpir	[31]
Plasmids
pRE112	Suicide vector Cm^R SacB oriT oriV	[31]
p-ldh A’	Cm^R, pRE112 derivative, where a 625-bp DNA fragment containing the ldhA’ was inserted	This work
p-adh E’	Cm^R, pRE112 derivative, where a 872-bp DNA fragment containing the adhE’ was inserted	This work
p-pta’	Cm^R, pRE112 derivative, where a 901-bp DNA fragment containing the pta’ was inserted	This work

ISSN: 2731-3654