Skip to main content

Advertisement

Table 5 Primers used in this work

From: Effect of the inactivation of lactate dehydrogenase, ethanol dehydrogenase, and phosphotransacetylase on 2,3-butanediol production in Klebsiella pneumoniae strain

Primer name Primer sequences
ldh A’-up 5′-CGGGGTACC ACGGTTGCGAACGGTATGTA-3′(Kpn I)
ldh A’-down 5′-CGAGCTC AGTGGTCTCCGAAATGCTGA-3′(Sac I)
adh E’-up 5′-CATGCATGC ACCATCGTACGTAA AGGTGC-3′(Sph I)
adh E’-down 5′-CGAGCTC TTCGGAATACCCAGCTCAGC-3′(Sac I)
pta’ -up 5′-CATGCATGC CAACTACATCAACGCCGACTGG-3′(SphI)
pta’-down 5′-CGAGCTC CGCTTTGTACGTGGTGTTACCG-3′(SacI)
cat-up 5′-CGGGCCCTAAATACCTGTGACGGAAGAT-3′
cat-down 5′-ATCGGGCCCTATCACTTATTCAGGCGTAGC-3′
A-down 5′-TACAAAACCAGCACCGTCCCT-3′
P-down 5′-CGCTGTGGATGACCCGCAACG-3′
L-down 5′-CCGTCCGACACTTTACCTTCC-3′