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Table 5 Primers used in this work

From: Effect of the inactivation of lactate dehydrogenase, ethanol dehydrogenase, and phosphotransacetylase on 2,3-butanediol production in Klebsiella pneumoniae strain

Primer name

Primer sequences

ldh A’-up

5′-CGGGGTACC ACGGTTGCGAACGGTATGTA-3′(Kpn I)

ldh A’-down

5′-CGAGCTC AGTGGTCTCCGAAATGCTGA-3′(Sac I)

adh E’-up

5′-CATGCATGC ACCATCGTACGTAA AGGTGC-3′(Sph I)

adh E’-down

5′-CGAGCTC TTCGGAATACCCAGCTCAGC-3′(Sac I)

pta’ -up

5′-CATGCATGC CAACTACATCAACGCCGACTGG-3′(SphI)

pta’-down

5′-CGAGCTC CGCTTTGTACGTGGTGTTACCG-3′(SacI)

cat-up

5′-CGGGCCCTAAATACCTGTGACGGAAGAT-3′

cat-down

5′-ATCGGGCCCTATCACTTATTCAGGCGTAGC-3′

A-down

5′-TACAAAACCAGCACCGTCCCT-3′

P-down

5′-CGCTGTGGATGACCCGCAACG-3′

L-down

5′-CCGTCCGACACTTTACCTTCC-3′