Figure 2

Schematic diagram of a biofilm reactor coupled to fixed-bed adsorption. The KA-I resin was packed in glass columns of 250-mm length and 30-mm diameter. Approximately 50 to 60 g of the resin was loaded in each column. After autoclaving, the resin column was connected to a 2-L biofilm reactor to construct an online product removal system. With feeding of concentrated P2 medium, fed-batch fermentation can be performed. Operation details are described in the Methods section. SEM, scanning electron microscopy; T, temperature; P, pressure.