Figure 1

Glycome profiling of untreated P. trichocarpa (Poplar), P. virgatum (Switchgrass), A. americana leaves (AAL), A. salmiana leaves (ASL), A. tequilana leaves (ATL), and A. americana heart (AAH) biomasses. Sequentially extracted materials released from each biomass sample by various reagents (as labeled at the bottom of each map) were loaded onto the ELISA plates and screened against an array of plant glycan-directed monoclonal antibodies. The legend panel on the right displays the nature of the polysaccharides predominantly recognized by these monoclonal antibodies (mAbs). Antibody binding is represented as colored heat maps, with black signifying no binding, and light yellow representing the strongest binding. The bar graphs at the top indicate the amount of material recovered at each extraction step per gram of alcohol insoluble residue (AIR). AAL: A. americana leaves; ASL: A. salmiana leaves; ATL: A. tequilana leaves; AAH: A. americana heart; AG: Arabinogalactan; HG: Homogalacturonan; KOH: Potassium Hydroxide; PC: Post Chlorite; RG: Rhamnogalacturonan; XG: Xyloglucan.