Figure 2

Sugar yield data from enzymatic hydrolysis of (a,c,d) non-pretreated (b) extractives free non-pretreated and (e-h) hydrothermal-pretreated P. trichocarpa (Poplar), P. virgatum (Switchgrass: SG), A. americana leaves (AAL), A. salmiana leaves (ASL), A. tequilana leaves (ATL), and A. americana heart (AAH) biomasses. Biomass samples were digested with cellulase supplemented with xylanase and pectinase as described in the Materials and Method Section: (a,b,e-h) 72 hours hydrolysis using 150 mg protein/g structural sugar enzyme loading, (c) 72 hours hydrolysis using 15 mg protein/g structural sugar enzyme loading, d) 144 hours hydrolysis using 15 mg protein/g structural sugar enzyme loading. Hydrothermal pretreatment conditions are described in Supporting Information S4. Pretreatment conditions 105 to 3.0, for example, represents pretreatment at 105°C with a severity factor of 3.0; and NP represents no pretreatment. Yields reflect the amount of sugar released of the maximum available in raw biomass. Error bars represent standard deviation of quadruplicates. AAL: A. americana leaves; ASL: A. salmiana leaves; ATL: A. tequilana leaves; AAH: A. americana heart; NP: Non-pretreated; SG: Switchgrass.