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Table 2 Effect of accessory enzymes on release of Glc from AHP-pretreated LQ by the 8-component synthetic enzyme mixture

From: Factors contributing to the recalcitrance of herbaceous dicotyledons (forbs) to enzymatic deconstruction

 

Glc yield, % of maximum

8-component alone

29.66 ± 0.74

+ Cip1

23.35 ± 3.15

+ Cip2

29.68 ± 0.03

+ Abf1

29.86 ± 0.51

+ Abf2

29.95 ± 0.28

+ AbfB

29.83 ± 0.69

+ XG

35.33 ± 0.34

+ GH12

27.48 ± 0.38

+ β-galactosidase

30.02 ± 1.76

  1. Values are the means ± 1 SD, n =3. The 8-component mixture was used at 15 mg/gm glucan and each accessory enzyme was added at 5 mg/gm glucan.
  2. Abf1, arabinosidase 1; Abf2, arabinosidase 2; AbfB, arabinosidase B; AHP, alkaline hydrogen peroxide; Cip1, cellulose-inducible protein 1; Cip2, cellulose-inducible protein 2; GH12, Cel12A; Glc, total glucose; LQ, lamb’s quarter; XG, xyloglucanase.