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Table 2 Effect of accessory enzymes on release of Glc from AHP-pretreated LQ by the 8-component synthetic enzyme mixture

From: Factors contributing to the recalcitrance of herbaceous dicotyledons (forbs) to enzymatic deconstruction

  Glc yield, % of maximum
8-component alone 29.66 ± 0.74
+ Cip1 23.35 ± 3.15
+ Cip2 29.68 ± 0.03
+ Abf1 29.86 ± 0.51
+ Abf2 29.95 ± 0.28
+ AbfB 29.83 ± 0.69
+ XG 35.33 ± 0.34
+ GH12 27.48 ± 0.38
+ β-galactosidase 30.02 ± 1.76
  1. Values are the means ± 1 SD, n =3. The 8-component mixture was used at 15 mg/gm glucan and each accessory enzyme was added at 5 mg/gm glucan.
  2. Abf1, arabinosidase 1; Abf2, arabinosidase 2; AbfB, arabinosidase B; AHP, alkaline hydrogen peroxide; Cip1, cellulose-inducible protein 1; Cip2, cellulose-inducible protein 2; GH12, Cel12A; Glc, total glucose; LQ, lamb’s quarter; XG, xyloglucanase.