Combined substrate, enzyme and yeast feed in simultaneous saccharification and fermentation allow bioethanol production from pretreated spruce biomass at high solids loadings

Table 1 Composition of spruce slurry and non-washed solids used in simultaneous saccharification and fermentation (SSF)

Components	Concentration when slurry was used, g kg^-1*	Concentration when non-washed solids were used, g kg^-1*
WIS	200	200
Glucan	106	106
Glucose	24.8	14.5
Mannose	24.6	14.4
Galactose	4.9	2.9
Xylose	11.9	6.9
HMF	2.8	1.7
Furfural	2.3	1.3
Acetic acid	5.7	3.3

*Concentrations correspond to a total water insoluble solids (WIS) content of 20% (w/w) in SSF with a total working weight of 1.5 kg. HMF, 5-hydroxy-methyl furfural.

ISSN: 2731-3654