Figure 4From: Quantitative dynamics of triacylglycerol accumulation in microalgae populations at single-cell resolution revealed by Raman microspectroscopyQuantitative analysis of TAG content and lipid unsaturation degree in single cells. (A) TAG content of individual cells as predicted by PLSR. Each square represents one Group N- cell. (B) Lipid unsaturation degree as determined by I1656/I1441. Each triangle represents one Group N + cell, and each square represents one Group N- cell. (C) Distribution of TAG content of Group N- cells at each time point. X axis is the predicted TAG content (mg g−1 dry weight), Y axis is the percentage of cells. Since TAG content is increasing sharply, the range of X axis is different between time points. (D) Distribution of lipid unsaturation degree of Group N- or Group N + cells at each time point. X axis is the lipid unsaturation degree, Y axis is the percentage of cells. h: hours; PLSR: partial least square regression; TAG: triacylglycerol.Back to article page