Quantitative analysis of TAG content and lipid unsaturation degree in single cells. (A) TAG content of individual cells as predicted by PLSR. Each square represents one Group N- cell. (B) Lipid unsaturation degree as determined by I1656/I1441. Each triangle represents one Group N + cell, and each square represents one Group N- cell. (C) Distribution of TAG content of Group N- cells at each time point. X axis is the predicted TAG content (mg g−1 dry weight), Y axis is the percentage of cells. Since TAG content is increasing sharply, the range of X axis is different between time points. (D) Distribution of lipid unsaturation degree of Group N- or Group N + cells at each time point. X axis is the lipid unsaturation degree, Y axis is the percentage of cells. h: hours; PLSR: partial least square regression; TAG: triacylglycerol.