Figure 4
From: Production of free monounsaturated fatty acids by metabolically engineered Escherichia coli
Effects of fadD knockout on free fatty-acid production. BL21/pE-AtFatAssi2, strain BL21 star(DE3) expressing Arabidopsis thioesterase AtFatA and fatty acid desaturase SSI2; BL21ΔfadD/pE-AtFatAssi2, knockout of native fadD while expressing AtFatA and SSI2; 16:1, palmitoleate; 16:0, palmitate; 18:1, cis-vaccenate. Data were obtained after each strain was induced for 16 h in liquid M9 minimal medium supplemented with 1 mM MgSO4 and 20 g/L glucose. FFA, free fatty acid; OD, optical density.