Table 4 Strains and plasmids used in this study
From: Production of free monounsaturated fatty acids by metabolically engineered Escherichia coli
Strains or plasmids | Genotype/description | Sources |
|---|---|---|
Strains | ||
E. coli BL21 star(DE3) | F−ompT hsdS B (rB− mB−) gal dcm rne131 (DE3) | Invitrogen |
E. coli BL21 star(DE3) ΔfadD | Knockout of fadD encoding acyl-CoA synthetase | This study |
Plasmids | ||
pACYCduet-1 | Cm r oriP15A lacI q T7p | Novagen |
pET30a | Kan r oripBR322 lacI q T7p | Novagen |
pEASY-Blunt | Kan r Amp r oripUC | Transgen |
pE-ssi2 | pET30a harboring Arabidopsis fatty-acid desaturase | [59] |
pA-acc | pACYCduet-1 harboring E. coli acetyl-CoA carboxylase | [60] |
PEASY-AtFatA | pEASY-Blunt harboring Arabidopsis thioesterase | This study |
pA-AtFatA | pACYCduet-1 harboring Arabidopsis thioesterase | This study |
pE-AtFatAssi2 | pET30a harboring Arabidopsis fatty-acid desaturase and thioesterase | This study |
pKD46 | AproriR101 repA(Ts) λ Red (γ, β and exo) | Coli Genetic Stock Center |
pKD4 | FRT-Kan r -FRT oriR6K | Coli Genetic Stock Center |
pCP20 | AprCmrrepA(Ts) FLP | Coli Genetic Stock Center |