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Table 4 Strains and plasmids used in this study

From: Production of free monounsaturated fatty acids by metabolically engineered Escherichia coli

Strains or plasmids Genotype/description Sources
Strains
E. coli BL21 star(DE3) FompT hsdS B (rB mB) gal dcm rne131 (DE3) Invitrogen
E. coli BL21 star(DE3) ΔfadD Knockout of fadD encoding acyl-CoA synthetase This study
Plasmids
pACYCduet-1 Cm r oriP15A lacI q T7p Novagen
pET30a Kan r oripBR322 lacI q T7p Novagen
pEASY-Blunt Kan r Amp r oripUC Transgen
pE-ssi2 pET30a harboring Arabidopsis fatty-acid desaturase [59]
pA-acc pACYCduet-1 harboring E. coli acetyl-CoA carboxylase [60]
PEASY-AtFatA pEASY-Blunt harboring Arabidopsis thioesterase This study
pA-AtFatA pACYCduet-1 harboring Arabidopsis thioesterase This study
pE-AtFatAssi2 pET30a harboring Arabidopsis fatty-acid desaturase and thioesterase This study
pKD46 AproriR101 repA(Ts) λ Red (γ, β and exo) Coli Genetic Stock Center
pKD4 FRT-Kan r -FRT oriR6K Coli Genetic Stock Center
pCP20 AprCmrrepA(Ts) FLP Coli Genetic Stock Center